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Fasciola hepatica-Pseudosuccinea columella interaction: effect of increasing parasite doses, successive exposures and geographical origin on the infection outcome of susceptible and naturally-resistant snails from Cuba ArchiMer
Alba, Annia; Vazquez, Antonio A.; Sanchez, Jorge; Duval, David; Hernandez, Hilda M.; Sabourin, Emeline; Vittecoq, Marion; Hurtrez-bousses, Sylvie; Gourbal, Benjamin.
Background: Pseudosuccinea columella is one of the most widespread vectors of Fasciola hepatica, a globally distributed trematode that affects humans, livestock and wildlife. The exclusive occurrence in Cuba of susceptible and naturally-resistant populations to F. hepatica within this snail species, offers a fascinating model for evolutionary biology, health sciences and vector control strategies. In particular, resistance in P. columella is characterized by the encapsulation of the parasite by host's immune cells and has been experimentally tested using different Cuban F. hepatica isolates with no records of successful infection. Here, we aimed to explore for the first time, the effect of different parasite doses, successive exposures and different...
Tipo: Text Palavras-chave: Snail-trematode interaction; Lymnaeidae; Liver fluke; Experimental infection; Immune priming; Compatibility; Allopatric parasites.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00467/57894/71760.pdf
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Immune priming and portal of entry effectors improve response to vibrio infection in a resistant population of the European abalone ArchiMer
Dubief, Bruno; Nunes, Flavia; Basuyaux, Olivier; Paillard, Christine.
Since 1997, populations of the European abalone Haliotis tuberculata suffer mass mortalities attributed to the bacterium Vibrio harveyi. These mortalities occur at the spawning season, when the abalone immune system is depressed, and when temperatures exceed 17 °C, leading to favorable conditions for V. harveyi proliferation. In order to identify mechanisms of disease resistance, experimental successive infections were carried out on two geographically distinct populations: one that has suffered recurrent mortalities (Saint-Malo) and one that has not been impacted by the disease (Molène). Furthermore, abalone surviving these two successive bacterial challenges and uninfected abalone were used for several post-infection analyses. The Saint-Malo population...
Tipo: Text Palavras-chave: Immunity; Hemocyte; Abalone; Disease; Extracellular products; Immune priming; Vibrio harveyi; Flow cytometry; Resistance; Phagocytosis; Bacterial growth; QPCR; Gill.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00357/46800/46667.pdf
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